NanoBRET® Target Engagement CDK Selectivity Systems
Profile Compound Selectivity for Cyclin-Dependent Kinases (CDKs) in Live Cells
- Determine compound or inhibitor affinity and occupancy against up to 16 CDKs
- Each NanoBRET® TE CDK Selectivity System uses a simple, addition-only microplate workflow and provides a CDK Vector Panel (A or B) and a NanoBRET® TE Kinase Assay
- Two transfection-ready NanoBRET® TE CDK vector panels (A and B) with 8 individual pairings of CDK-NanoLuc® fusion vector with cyclin/carrier DNA
Catalog Number:
Choose a product
Catalog Number: NP5000
Catalog Number: NP5050
Catalog Number: NP5100
Catalog Number: NP5150
Profile Compound Selectivity Against 16 CDKs in Live Cells
The NanoBRET® Target Engagement (TE) CDK Selectivity Systems quantitatively measure intracellular compound affinity and occupancy against two panels of 8 CDKs each, allowing you to measure up to 16 CDKs in a single experiment. Each vector panel contains 8 individual tubes of CDK-NanoLuc® fusion vectors that are premixed with either a cyclin expression vector or carrier DNA for optimal transfections.
The CDK Selectivity Systems rely on the NanoBRET® TE technology, a bioluminescence resonance energy transfer (BRET) method that uses the small, extremely bright NanoLuc® luciferase as the energy donor and a cell-permeable NanoBRET® TE Tracer as the energy acceptor. The CDK Selectivity Systems use an addition-only, multiwell plate format with a single NanoBRET® TE Kinase Assay.
How the NanoBRET® TE CDK Selectivity Systems Work
A CDK-NanoLuc® fusion vector is co-transfected with a cyclin expression vector into cells. Within the cells, the co-expression drives association of the CDK and cyclin, creating a biased readout specific for the CDK partner protein pair. BRET is achieved by luminescent energy transfer from NanoLuc® luciferase to the cell-permeable NanoBRET® TE Tracer that is bound to the CDK-NanoLuc® fusion protein. When the test compound is added, it competes with the tracer. This results in a dose-dependent decrease in NanoBRET® signal, allowing intracellular affinity of the test compound to be quantitated.
Day 1 - Transfect Cells
Day 1 - Transfect Cells
Day 2 - Perform Assay
Day 2 - Perform Assay
Day 2 - Acquire Data
Day 2 - Acquire Data
Ready-to-Use Panels Simplify Cellular CDK Profiling
The NanoBRET® TE CDK Selectivity Systems simplify routine selectivity screening with ready-to-use CDK Vector Panels. Each panel contains 8 individual tubes of CDK-NanoLuc® fusion vectors that are premixed with either a cyclin expression vector or carrier DNA for optimal transfection (see tables). Just thaw, dilute and transfect these consumable vector mixes. Panel A contains the highly studied CDKs, and Panel B contains the understudied CDKs.
Turn-Key Cellular CDK Profiling System
The NanoBRET® TE CDK Selectivity Systems contain a NanoBRET® TE CDK Vector Panel (A or B) and a single NanoBRET® TE Intracellular Kinase Assay, K-10. The optimized assay protocol offers an easy, addition-only workflow. Begin by simply transfecting the vector panel into HEK293 cells in a multiwell plate. Following overnight incubation, add your test compounds to the assay plate and run the NanoBRET® TE Kinase Assay, K-10, using a single, optimized concentration of NanoBRET® Tracer K-10 for each CDK. A plate reader is used to measure BRET.
Get the Data You Need
Determine intracellular affinity and compound fractional occupancy with the NanoBRET® TE CDK Selectivity Assays.
The CDK4/6 inhibitors Palbociclib (Panel A and table) and Ribociclib (table) showed very selective engagement of CDK4/6, while Abemaciclib (Panel B and table) was CDK4/6-selective but also engaged CDK9 and the TAIRE family (table). Other inhibitors potently engaged CDK4/6 but also exhibited collateral engagement of other CDKs, including the TAIRE family. Affinities of >1µM are not reported in the table, although they are measurable as shown (Panel A).
Determine Compound Fractional Occupancy
The NanoBRET® TE CDK Selectivity Assays can quantitate the fractional occupancy of compound for each CDK.
Quantitative fractional occupancy measurements for Palbociclib and Abemaciclib. Measurements were obtained by including the appropriate experimental controls, so the BRET ratio can be converted to occupancy. Fractional occupancy enables profiling compound selectivity across the CDKs at a single compound concentration. Palbociclib and Abemaciclib are CDK4/6-selective inhibitors. The occupancy data for Palbociclib at 500nM and Abemaciclib at 500nM against CDK Vector Panel A and CDK Vector Panel B are shown (Mean and Standard Deviation were calculated from 3 independent experiments).
Featured Publication
In this study, the NanoBRET® TE method was used to profile a collection of 46 clinically advanced CDK inhibitors and tool molecules in live cells. The results confirmed the binding of advanced clinical compounds and revealed new drug-CDK interactions. These new findings offer the potential to repurpose drugs for lesser-studied CDK family members. This paper highlights the importance of determining drug selectivity across the CDK family in live cells. The NanoBRET® TE CDK Selectivity Systems expanded on this work, simplifying the workflow to use a single NanoBRET® TE Assay (K-10) and ready-to-use CDK Vector Panels.
Wells, C.I. et al. (2020) Quantifying CDK inhibitor selectivity in live cells. Nat. Comm. 11, 2743.
Protocols
Specifications
Catalog Number:
What's in the box?
| Item | Part # | Size |
|---|---|---|
CDK1/CCNB1 Vector Mix |
NP501A | 1 × 120μl |
CDK2/CCNE1 Vector Mix |
NP502A | 1 × 120μl |
CDK3/CCNE1 Vector Mix |
NP503A | 1 × 120μl |
CDK4/CCND3 Vector Mix |
NP504A | 1 × 120μl |
CDK5/CDK5R1 Vector Mix |
NP505A | 1 × 120μl |
CDK6/CCDN1 Vector Mix |
NP506A | 1 × 120μl |
CDK7/Carrier Vector Mix |
NP507A | 1 × 120μl |
CDK9/CCNK Vector Mix |
NP508A | 1 × 120μl |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
NanoBRET® TE Selectivity Vector Panel Limited Use Label License
BY USE OF THIS PRODUCT, USER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If user is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide user with a full refund.
User may use this product for research use only; however, no amplification or isolation of the vector components of this product and no transfer or commercial use of this product is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to: (1) use in further product manufacture; and (2) resale of the product, whether or not such product is resold for use in research. User shall have no right to manufacture or have a third party manufacture the vector components within this product for any purpose. User shall have no right to modify or otherwise create variations of the nucleotide sequence of the luciferase gene. Notwithstanding the foregoing, user may use this product in provision of services to third parties in exchange for consideration; however, user may not transfer the product to third parties. No other use of this product is authorized without the prior express written consent of Promega.
In addition, user must:
(1a) use Nano-Glo®-branded luminescent assay reagents (LARs) for all determinations of luminescence activity of this product; or
(1b) contact Promega to obtain a license for use of the product with LARs not manufactured by Promega.
For uses of this product for energy transfer (such as bioluminescence resonance energy transfer), user must:
(2a) use NanoBRET®-branded energy acceptors (e.g., NanoBRET® tracers, NanoBRET® dyes, BRET-optimized HaloTag® ligands) for all determinations of energy transfer activity; and
(2b) use NanoBRET®-branded LARs (e.g., NanoBRET® Nano-Glo® Substrate) or Intracellular TE Nano-Glo® Substrate/Inhibitor or Intracellular TE Nano-Glo® Vivazine/Inhibitor for all determinations of luminescent activity; or
(2c) contact Promega to obtain a license for use of the product for energy transfer assays using energy acceptors not manufactured by Promega. No license is needed if the energy transfer acceptor is a genetically encoded autofluorescent protein.
With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
U.S. Pat. Nos. 8,557,970, 8,669,103, 9,777,311, 9,840,730, 9,951,373, 10,233,485, 10,633,690, 10,774,364, 10,844,422, 11,365,436, 11,661,623, 11,667,950; European Pat. Nos. 2456864, 2635595, 2990478, 3181687, 3409764; Japanese Pat. Nos. 6038649, 6155424, 6227615, 6374420, 6539689; and other patents and patents pending.
What's in the box?
| Item | Part # | Size | Concentration | Available Separately |
|---|---|---|---|---|
Transfection Carrier DNA |
E488B | 1 × 100μg | View Product | |
NanoBRET® Nano-Glo® Substrate |
N157C | 1 × 330μl | View Product | |
Tracer Dilution Buffer |
N219A | 1 × 5ml | View Product | |
Extracellular NanoLuc® Inhibitor |
N235B | 1 × 110μl | View Product | |
NanoBRET® Tracer K-10 |
N264B | 1 × 300μl | 400μM | |
LIMK1-NanoLuc® Fusion Vector |
NV339A | 1 × 20μg | 1μg/μl | |
CDK1/CCNB1 Vector Mix |
NP501A | 1 × 120μl | ||
CDK2/CCNE1 Vector Mix |
NP502A | 1 × 120μl | ||
CDK3/CCNE1 Vector Mix |
NP503A | 1 × 120μl | ||
CDK4/CCND3 Vector Mix |
NP504A | 1 × 120μl | ||
CDK5/CDK5R1 Vector Mix |
NP505A | 1 × 120μl | ||
CDK6/CCDN1 Vector Mix |
NP506A | 1 × 120μl | ||
CDK7/Carrier Vector Mix |
NP507A | 1 × 120μl | ||
CDK9/CCNK Vector Mix |
NP508A | 1 × 120μl |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.NanoBRET® TE Selectivity Vector Panel Limited Use Label License
BY USE OF THIS PRODUCT, USER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If user is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide user with a full refund.
User may use this product for research use only; however, no amplification or isolation of the vector components of this product and no transfer or commercial use of this product is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to: (1) use in further product manufacture; and (2) resale of the product, whether or not such product is resold for use in research. User shall have no right to manufacture or have a third party manufacture the vector components within this product for any purpose. User shall have no right to modify or otherwise create variations of the nucleotide sequence of the luciferase gene. Notwithstanding the foregoing, user may use this product in provision of services to third parties in exchange for consideration; however, user may not transfer the product to third parties. No other use of this product is authorized without the prior express written consent of Promega.
In addition, user must:
(1a) use Nano-Glo®-branded luminescent assay reagents (LARs) for all determinations of luminescence activity of this product; or
(1b) contact Promega to obtain a license for use of the product with LARs not manufactured by Promega.
For uses of this product for energy transfer (such as bioluminescence resonance energy transfer), user must:
(2a) use NanoBRET®-branded energy acceptors (e.g., NanoBRET® tracers, NanoBRET® dyes, BRET-optimized HaloTag® ligands) for all determinations of energy transfer activity; and
(2b) use NanoBRET®-branded LARs (e.g., NanoBRET® Nano-Glo® Substrate) or Intracellular TE Nano-Glo® Substrate/Inhibitor or Intracellular TE Nano-Glo® Vivazine/Inhibitor for all determinations of luminescent activity; or
(2c) contact Promega to obtain a license for use of the product for energy transfer assays using energy acceptors not manufactured by Promega. No license is needed if the energy transfer acceptor is a genetically encoded autofluorescent protein.
With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
NanoBRET® TE Tracers Limited Use Label License
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
Researcher may use this product for research use only; no commercial use is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product, whether or not such product is resold for use in research. Researcher shall have no right to modify or otherwise create variations of the product. No other use or transfer of this product is authorized without the prior express written consent of Promega.
For uses of this product for energy transfer (such as bioluminescence resonance energy transfer), researcher must:
(i) use NanoLuc®-branded luciferase or derivatives for all energy transfer determinations conducted with this product; and
(ii) use NanoBRET®-branded luminescent assay reagents (LARs; e.g., NanoBRET® Nano-Glo® Substrate), Intracellular TE Nano-Glo® Substrate/Inhibitor or Intracellular TE Nano-Glo® Vivazine®/Inhibitor for all determinations of luminescent activity; or
(iii) contact Promega to obtain a license for use of the product with LARs not listed above or manufactured by Promega.
In addition, researcher must:
Contact Promega to obtain a license for use of the product in assays not involving bioluminescence resonance energy transfer (BRET).
With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
NanoBRET® TE LAR Limited Use Label License
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
Researcher may use this product for research use only; no commercial use is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product, whether or not such product is resold for use in research. Researcher shall have no right to modify or otherwise create variations of the product. No other use or transfer of this product is authorized without the prior express written consent of Promega.
For uses of this product intended for energy transfer (such as bioluminescence resonance energy transfer) to acceptors other than genetically encoded autofluorescent protein, researcher must:
(i) use NanoBRET®-branded energy acceptors (e.g., NanoBRET® tracers, NanoBRET® dyes, BRET-optimized HaloTag® ligands) for all determinations of energy transfer activity; or
(ii) contact Promega to obtain a license for use of the product for energy transfer assays using energy acceptors not manufactured by Promega.
With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
NanoLuc® ORF TE Vector Limited Use Label License
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
Researcher may use this product for research use only; no transfer or commercial use of this product is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product, whether or not such product is resold for use in research. Researcher shall have no right to modify or otherwise create variations of the nucleotide sequence of the luciferase gene. No other use of this product is authorized without the prior express written consent of Promega.
In addition, researcher must:
(1a) use Nano-Glo®-branded luminescent assay reagents (LARs) for all determinations of luminescence activity of this product; or
(1b) contact Promega to obtain a license for use of the product with LARs not manufactured by Promega.
For uses of this product (NanoLuc® ORF Vector) for energy transfer (such as bioluminescence resonance energy transfer), researcher must:
(2a) use NanoBRET®-branded luminescent assay reagents (LARs; e.g., NanoBRET® Nano-Glo® Substrate), Intracellular TE Nano-Glo® Substrate/Inhibitor or Intracellular TE Nano-Glo® Vivazine®/Inhibitor for all determinations of luminescent activity; and
(2b) use NanoBRET®-branded energy acceptors (e.g., NanoBRET® tracers, NanoBRET® dyes, BRET-optimized HaloTag® ligands) for all determinations of energy transfer activity; or
(2c) contact Promega to obtain a license for use of the product for energy transfer assays using energy acceptors not manufactured by Promega. No license is needed if the energy transfer acceptor is a genetically encoded autofluorescent protein.
With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
U.S. Pat. Nos. 8,557,970, 8,669,103, 9,777,311, 9,840,730, 9,951,373, 10,233,485, 10,633,690, 10,774,364, 10,844,422, 11,365,436, 11,661,623, 11,667,950; European Pat. Nos. 2456864, 2635595, 2990478, 3181687, 3409764; Japanese Pat. Nos. 6038649, 6155424, 6227615, 6374420, 6539689; and other patents and patents pending.
U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.
U.S. Pat. No. 10,024,862; Japanese Pat. No. 7676487; and other patents and patents pending.
U.S. Pat. No. 10,513,694, European Pat. No. 3313846 and other patents.
What's in the box?
| Item | Part # | Size |
|---|---|---|
CDK10/CCNL2 Vector Mix |
NP511A | 1 × 120μl |
CDK14/CCNY Vector Mix |
NP512A | 1 × 120μl |
CDK15/CCNY Vector Mix |
NP513A | 1 × 120μl |
CDK16/CCNY Vector Mix |
NP514A | 1 × 120μl |
CDK17/CCNY Vector Mix |
NP515A | 1 × 120μl |
CDK18/CCNY Vector Mix |
NP516A | 1 × 120μl |
CDKL2/Carrier Vector Mix |
NP517A | 1 × 120μl |
CDK20/CCNH Vector Mix |
NP518A | 1 × 120μl |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
NanoBRET® TE Selectivity Vector Panel Limited Use Label License
BY USE OF THIS PRODUCT, USER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If user is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide user with a full refund.
User may use this product for research use only; however, no amplification or isolation of the vector components of this product and no transfer or commercial use of this product is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to: (1) use in further product manufacture; and (2) resale of the product, whether or not such product is resold for use in research. User shall have no right to manufacture or have a third party manufacture the vector components within this product for any purpose. User shall have no right to modify or otherwise create variations of the nucleotide sequence of the luciferase gene. Notwithstanding the foregoing, user may use this product in provision of services to third parties in exchange for consideration; however, user may not transfer the product to third parties. No other use of this product is authorized without the prior express written consent of Promega.
In addition, user must:
(1a) use Nano-Glo®-branded luminescent assay reagents (LARs) for all determinations of luminescence activity of this product; or
(1b) contact Promega to obtain a license for use of the product with LARs not manufactured by Promega.
For uses of this product for energy transfer (such as bioluminescence resonance energy transfer), user must:
(2a) use NanoBRET®-branded energy acceptors (e.g., NanoBRET® tracers, NanoBRET® dyes, BRET-optimized HaloTag® ligands) for all determinations of energy transfer activity; and
(2b) use NanoBRET®-branded LARs (e.g., NanoBRET® Nano-Glo® Substrate) or Intracellular TE Nano-Glo® Substrate/Inhibitor or Intracellular TE Nano-Glo® Vivazine/Inhibitor for all determinations of luminescent activity; or
(2c) contact Promega to obtain a license for use of the product for energy transfer assays using energy acceptors not manufactured by Promega. No license is needed if the energy transfer acceptor is a genetically encoded autofluorescent protein.
With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
U.S. Pat. Nos. 8,557,970, 8,669,103, 9,777,311, 9,840,730, 9,951,373, 10,233,485, 10,633,690, 10,774,364, 10,844,422, 11,365,436, 11,661,623, 11,667,950; European Pat. Nos. 2456864, 2635595, 2990478, 3181687, 3409764; Japanese Pat. Nos. 6038649, 6155424, 6227615, 6374420, 6539689; and other patents and patents pending.
What's in the box?
| Item | Part # | Size | Concentration | Available Separately |
|---|---|---|---|---|
Transfection Carrier DNA |
E488B | 1 × 100μg | View Product | |
NanoBRET® Nano-Glo® Substrate |
N157C | 1 × 330μl | View Product | |
Tracer Dilution Buffer |
N219A | 1 × 5ml | View Product | |
Extracellular NanoLuc® Inhibitor |
N235B | 1 × 110μl | View Product | |
NanoBRET® Tracer K-10 |
N264B | 1 × 300μl | 400μM | |
LIMK1-NanoLuc® Fusion Vector |
NV339A | 1 × 20μg | 1μg/μl | |
CDK10/CCNL2 Vector Mix |
NP511A | 1 × 120μl | ||
CDK14/CCNY Vector Mix |
NP512A | 1 × 120μl | ||
CDK15/CCNY Vector Mix |
NP513A | 1 × 120μl | ||
CDK16/CCNY Vector Mix |
NP514A | 1 × 120μl | ||
CDK17/CCNY Vector Mix |
NP515A | 1 × 120μl | ||
CDK18/CCNY Vector Mix |
NP516A | 1 × 120μl | ||
CDKL2/Carrier Vector Mix |
NP517A | 1 × 120μl | ||
CDK20/CCNH Vector Mix |
NP518A | 1 × 120μl |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.NanoBRET® TE Selectivity Vector Panel Limited Use Label License
BY USE OF THIS PRODUCT, USER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If user is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide user with a full refund.
User may use this product for research use only; however, no amplification or isolation of the vector components of this product and no transfer or commercial use of this product is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to: (1) use in further product manufacture; and (2) resale of the product, whether or not such product is resold for use in research. User shall have no right to manufacture or have a third party manufacture the vector components within this product for any purpose. User shall have no right to modify or otherwise create variations of the nucleotide sequence of the luciferase gene. Notwithstanding the foregoing, user may use this product in provision of services to third parties in exchange for consideration; however, user may not transfer the product to third parties. No other use of this product is authorized without the prior express written consent of Promega.
In addition, user must:
(1a) use Nano-Glo®-branded luminescent assay reagents (LARs) for all determinations of luminescence activity of this product; or
(1b) contact Promega to obtain a license for use of the product with LARs not manufactured by Promega.
For uses of this product for energy transfer (such as bioluminescence resonance energy transfer), user must:
(2a) use NanoBRET®-branded energy acceptors (e.g., NanoBRET® tracers, NanoBRET® dyes, BRET-optimized HaloTag® ligands) for all determinations of energy transfer activity; and
(2b) use NanoBRET®-branded LARs (e.g., NanoBRET® Nano-Glo® Substrate) or Intracellular TE Nano-Glo® Substrate/Inhibitor or Intracellular TE Nano-Glo® Vivazine/Inhibitor for all determinations of luminescent activity; or
(2c) contact Promega to obtain a license for use of the product for energy transfer assays using energy acceptors not manufactured by Promega. No license is needed if the energy transfer acceptor is a genetically encoded autofluorescent protein.
With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
NanoBRET® TE Tracers Limited Use Label License
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
Researcher may use this product for research use only; no commercial use is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product, whether or not such product is resold for use in research. Researcher shall have no right to modify or otherwise create variations of the product. No other use or transfer of this product is authorized without the prior express written consent of Promega.
For uses of this product for energy transfer (such as bioluminescence resonance energy transfer), researcher must:
(i) use NanoLuc®-branded luciferase or derivatives for all energy transfer determinations conducted with this product; and
(ii) use NanoBRET®-branded luminescent assay reagents (LARs; e.g., NanoBRET® Nano-Glo® Substrate), Intracellular TE Nano-Glo® Substrate/Inhibitor or Intracellular TE Nano-Glo® Vivazine®/Inhibitor for all determinations of luminescent activity; or
(iii) contact Promega to obtain a license for use of the product with LARs not listed above or manufactured by Promega.
In addition, researcher must:
Contact Promega to obtain a license for use of the product in assays not involving bioluminescence resonance energy transfer (BRET).
With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
NanoBRET® TE LAR Limited Use Label License
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
Researcher may use this product for research use only; no commercial use is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product, whether or not such product is resold for use in research. Researcher shall have no right to modify or otherwise create variations of the product. No other use or transfer of this product is authorized without the prior express written consent of Promega.
For uses of this product intended for energy transfer (such as bioluminescence resonance energy transfer) to acceptors other than genetically encoded autofluorescent protein, researcher must:
(i) use NanoBRET®-branded energy acceptors (e.g., NanoBRET® tracers, NanoBRET® dyes, BRET-optimized HaloTag® ligands) for all determinations of energy transfer activity; or
(ii) contact Promega to obtain a license for use of the product for energy transfer assays using energy acceptors not manufactured by Promega.
With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
NanoLuc® ORF TE Vector Limited Use Label License
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
Researcher may use this product for research use only; no transfer or commercial use of this product is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product, whether or not such product is resold for use in research. Researcher shall have no right to modify or otherwise create variations of the nucleotide sequence of the luciferase gene. No other use of this product is authorized without the prior express written consent of Promega.
In addition, researcher must:
(1a) use Nano-Glo®-branded luminescent assay reagents (LARs) for all determinations of luminescence activity of this product; or
(1b) contact Promega to obtain a license for use of the product with LARs not manufactured by Promega.
For uses of this product (NanoLuc® ORF Vector) for energy transfer (such as bioluminescence resonance energy transfer), researcher must:
(2a) use NanoBRET®-branded luminescent assay reagents (LARs; e.g., NanoBRET® Nano-Glo® Substrate), Intracellular TE Nano-Glo® Substrate/Inhibitor or Intracellular TE Nano-Glo® Vivazine®/Inhibitor for all determinations of luminescent activity; and
(2b) use NanoBRET®-branded energy acceptors (e.g., NanoBRET® tracers, NanoBRET® dyes, BRET-optimized HaloTag® ligands) for all determinations of energy transfer activity; or
(2c) contact Promega to obtain a license for use of the product for energy transfer assays using energy acceptors not manufactured by Promega. No license is needed if the energy transfer acceptor is a genetically encoded autofluorescent protein.
With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
U.S. Pat. Nos. 8,557,970, 8,669,103, 9,777,311, 9,840,730, 9,951,373, 10,233,485, 10,633,690, 10,774,364, 10,844,422, 11,365,436, 11,661,623, 11,667,950; European Pat. Nos. 2456864, 2635595, 2990478, 3181687, 3409764; Japanese Pat. Nos. 6038649, 6155424, 6227615, 6374420, 6539689; and other patents and patents pending.
U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.
U.S. Pat. No. 10,024,862; Japanese Pat. No. 7676487; and other patents and patents pending.
U.S. Pat. No. 10,513,694, European Pat. No. 3313846 and other patents.
U.S. Pat. Nos. 9,056,885, 10,139,400 and 10,215,751; European Pat. No. 2782916; and Japanese Pat. Nos. 6522339, 6574821 and 7227741.
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