Notes: Reliaprep RNA cell miniprep system was used to extract RNA from Hematopoietic stem cells derived from pluripotent stem cells. Then qRT-PCR analysis of miRNA and reverse transcription was performed using non-Promega products and 1 μg of RNA was used in a 20 μl GoScript reverse transcription reaction for the analysis of gene expression by qRT-PCR. (4975)

Notes: Total RNA was isolated from normal human diploid fibroblast TIG-3 cells with a ReliaPrep RNA Cell MiniPrep System. The isolated RNA was used to synthesize cRNA which was then purified using non-Promega products. Total RNA including miRNA was isolated with a ReliaPrep miRNA Cell and Tissue MiniPrep System. (4980)

Notes: Total RNA was isolated from HepG2, HLE, SK-HEP-1, MDA-MB-231 and MC7 cell lines using the ReliaPrep™ RNA Cell Miniprep System prior to cDNA synthesis and RT-qPCR. The Dual-Luciferase® Reporter Assay System was used to evaluate T-cell factor/lympocyte enhancer factor transcriptional activity. (4728)