Riboprobe® Systems
Produce High-Specific-Activity RNA Probes or Defined RNA Transcripts
- Systems available with SP6 RNA Polymerase, T7 RNA Polymerase or T3 RNA Polymerase
- SP6, T7 and T3 RNA Polymerases are extremely promoter-specific, allowing production of virtually homogeneous RNA using plasmid DNA as a template
- Allow synthesis of RNA corresponding to the coding or noncoding strand of cloned DNA from a single plasmid construct
Catalog Number:
Choose a system
Catalog Number: P1420
Catalog Number: P1430
Catalog Number: P1440
The Riboprobe® Systems are designed for in vitro preparation of high-specific-activity single-stranded RNA probes or microgram quantities of defined RNA transcripts from cloned DNA inserts. These systems contain all components necessary for in vitro transcription from a DNA template (excluding the radioisotope) and also contain RQ1 RNase-Free DNase (Cat.# M6101) for template removal following transcription.
Applications of the Riboprobe® Systems include:
- Generation of high-specific-activity RNA probes.
- Generation of microgram quantities of RNA transcripts.
- Generation of RNA processing substrates.
- Generation of antisense RNA.
Looking for a scalable IVT solution with animal-origin-free reagents? The RiboMAX™ AOF RNA Production System supports RNA synthesis from research to production.
Protocols
Specifications
Catalog Number:
What's in the box?
| Item | Part # | Size | Concentration | Available Separately |
|---|---|---|---|---|
|
RQ1 RNase-Free DNase |
M610C | 1 × 110u | View Product | |
|
Recombinant RNasin® Ribonuclease Inhibitor |
N251A | 1 × 2,500u | 40u/μl | View Product |
|
SP6 RNA Polymerase |
P108E | 1 × 500u | View Product | |
rATP |
P113A | 1 × 50μl | 10mM | View Product |
rCTP |
P114A | 1 × 50μl | 10mM | View Product |
rGTP |
P115A | 1 × 50μl | 10mM | View Product |
rUTP |
P116A | 1 × 50μl | 10mM | View Product |
|
DTT |
P117A | 1 × 100μl | 100mM | View Product |
|
Transcription Optimized 5X Buffer |
P118B | 1 × 500μl | View Product | |
|
Nuclease-Free Water |
P119A | 1 × 1,250μl | View Product | |
|
pGEM® Express Positive Control Template |
P256A | 1 × 5μg | View Product |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
What's in the box?
| Item | Part # | Size | Concentration | Available Separately |
|---|---|---|---|---|
|
RQ1 RNase-Free DNase |
M610C | 1 × 110u | View Product | |
|
Recombinant RNasin® Ribonuclease Inhibitor |
N251A | 1 × 2,500u | 40u/μl | View Product |
rCTP |
P114A | 1 × 50μl | 10mM | View Product |
rATP |
P113A | 1 × 50μl | 10mM | View Product |
rGTP |
P115A | 1 × 50μl | 10mM | View Product |
rUTP |
P116A | 1 × 50μl | 10mM | View Product |
|
DTT |
P117A | 1 × 100μl | 100mM | View Product |
|
Transcription Optimized 5X Buffer |
P118B | 1 × 500μl | View Product | |
|
Nuclease-Free Water |
P119A | 1 × 1,250μl | View Product | |
|
T3 RNA Polymerase |
P208A | 1 × 500u | View Product | |
|
pGEM® Express Positive Control Template |
P256A | 1 × 5μg | View Product |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
What's in the box?
| Item | Part # | Size | Concentration | Available Separately |
|---|---|---|---|---|
|
RQ1 RNase-Free DNase |
M610C | 1 × 110u | View Product | |
|
Recombinant RNasin® Ribonuclease Inhibitor |
N251A | 1 × 2,500u | 40u/μl | View Product |
rATP |
P113A | 1 × 50μl | 10mM | View Product |
rCTP |
P114A | 1 × 50μl | 10mM | View Product |
rGTP |
P115A | 1 × 50μl | 10mM | View Product |
rUTP |
P116A | 1 × 50μl | 10mM | View Product |
|
DTT |
P117A | 1 × 100μl | 100mM | View Product |
|
Transcription Optimized 5X Buffer |
P118B | 1 × 500μl | View Product | |
|
Nuclease-Free Water |
P119A | 1 × 1,250μl | View Product | |
|
T7 RNA Polymerase |
P207D | 1 × 500u | View Product | |
|
pGEM® Express Positive Control Template |
P256A | 1 × 5μg | View Product |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
Resources
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Generate high-specific-activity RNA probes or transcripts; choice of SP6/T7 or T3/T7 systems.
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Package of Transcription-Optimized Buffer, rNTPs and Nuclease-Free Water.
P1121
RiboMAXâ„¢ Large-Scale RNA Production Systems
Compatible with modified or unmodified nucleotides. Produces 2–5mg/ml RNA.
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T7 RiboMAXâ„¢ Express Large-Scale RNA Production System
Designed for speed. Produces 5–8.5mg/ml RNA in 30 minutes.
P1320
Frequently Used With
pGEM® Express Positive Control Template
Used to monitor in vitro transcription reactions with the Riboprobe® Systems.
P2561
PureYieldâ„¢ Plasmid Midiprep System
Up to 200µg of transfection-ready plasmid from 50ml cultures in 30 minutes.
A2492, A2495, A2496
Set of rATP, rCTP, rUTP, rGTP
Set of four rNTPs supplied at 10mM or 100mM.
P1221, E6000
T7 RNA Polymerase
DNA-dependent RNA polymerase for in vitro transcription that has high specificity for T7 promoter sequences.
P2075, P2077, P4074, P618X, P617X
