RNase ONEā¢ Ribonuclease
Degrades RNA to Cyclic Nucleotide Monophosphate (NMP) Intermediates
- Ability to cleave a phosphodiester bond between any two ribonucleotides
- Supplied at a concentration of 5ā10u/Ī¼l
- Intended for Laboratory Use (GPR) in the United States. Outside of the United States, this product is intended for research use only unless otherwise stated
Catalog Number:
Size
Catalog Number: M4261
Catalog Number: M4265
RNase ONEā¢ Ribonuclease is a 27kDa periplasmic enzyme from E. coli that catalyzes the degradation of RNA to cyclic nucleotide monophosphate (NMP) intermediates. Slower hydrolysis further catalyzes the degradation of these intermediates to 3Ā“-NMPs. RNase ONEā¢ Ribonuclease may be used to remove RNA from DNA preparations, for mismatch detection, RNase protection assays and mapping, or quantitation of RNA by selective cleavage of single-stranded regions. The enzyme is provided with 10X Reaction Buffer: 100mM Tris-HCl (pH 7.5 at 25Ā°C), 50mM EDTA, 2M sodium acetate.
References- Shen, V. and Schlessinger, D. (1982) The Enzymes, Vol. XV, 501.
- Meador, J., 3rd et al. (1990) Eur. J. Biochem. 187, 549ā53.
- Meador, J., 3rd and Kennell, D. (1990) Gene 95, 1ā7.
Storage Buffer: 10mM Tris-HCl (pH 8.0), 200mM NaCl, 50% (v/v) glycerol.
Source: Recombinant E. coli strain.
QC Tests: Activity, SDS-PAGE/purity, endonuclease, DNase.
Unit Definition: One unit is defined as the amount of enzyme required tocompletely degrade RNA at the rate of 100ng/second at 37Ā°C in 10mM Tris-HCl (pH 7.5 at 25Ā°C), 5mM EDTA, 200mM sodium acetate, 0.2Ī¼g/Ī¼l RNA, 0.05% NP-40 and 2Ī¼g/Ī¼l BSA.
Protocols
No protocols available
Specifications
Catalog Number:
What's in the box?
| Item | Part # | Size |
|---|---|---|
|
RNase ONEā¢ 10X Reaction Buffer |
M217A | 1 × 1ml |
|
RNase ONEā¢ Ribonuclease |
M426A | 1 × 1,000u |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.Storage Conditions
See Protocol for storage recommendations.
What's in the box?
| Item | Part # | Size |
|---|---|---|
|
RNase ONEā¢ 10X Reaction Buffer |
M217A | 5 × 1ml |
|
RNase ONEā¢ Ribonuclease |
M426C | 1 × 5,000u |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.Storage Conditions
See Protocol for storage recommendations.
Resources
No related resources available
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