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Food Control [epub ahead of print]. Evaluation of DNA extraction procedures for traceability of various tomato products. 2009

Turci, M., Sardaro, M.L.S., Visioli, G., Maestri, E., Marmiroli, M. and Marmiroli, N.

Notes: In this study, the authors wanted to examine the ability to trace the origin of tomato goods from fresh to processed. They tested several DNA extraction procedures for fresh tomato, tomato sauce, tomato puree, tomato pulp, whole peeled S. Marzano PDO (Protected Designation of Origin) tomato, whole peeled tomato, tomato concentrate and ‘‘Arrabbiata sauce”. Homogenized material (200mg) was extracted in three replicates using seven different methods including the Wizard® DNA Clean-Up System. The DNA extracted was then analyzed by agarose gel electrophoresis, quantified and tested in PCR using SSR loci. The authors concluded that the Wizard® DNA Clean-Up System was the most effective of the DNA extraction methods tested and yielded the greatest number of successful amplification reactions with lowest investment of personnel time and money. (4003)

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Clin. Can. Res. 12, 4515-22. Accumulation of promoter methylation suggests epigenetic progression in squamous cell carcinoma of the esophagus 2006

Guo, M., Ren, J., House, M.G., Qi, Y., Brock, M.V., Herman, J.G.

Notes: The authors studied multiple genes for aberrant DNA methylation in esophogeal cancer, to determine timing of methylation and epigenetic changes in early lesions. In addition the authors sought a role for preinvasive lesions in tumor initiation and progression. Tissues from esophageal cancers or various grades of esophogeal dysplasia were collected. A bisulfite modification was performed on genomic DNA to convert unmethylated cytosine to uracil in denatured DNA; methylated cytosines are resistant. The difference in reactivity to bisulfite treatment was used to provide unique sequences for primers to detect methylation differences. The resulting DNA samples were purified using the Wizard® DNA Clean-Up System. (4110)

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Int. Congr. Ser. 1239, 235–8. Portuguese population data on two pentanucleotide STR loci Penta E and Penta D. 2003

Ribeiro, T., Viriato, L., Vieira-Silva, C., Cruz, C., Espinheira, R. and Geada, H.

Notes: The authors reported population data for the Penta E and Penta D loci in 160 Portuguese Caucasians, 19 Portuguese of African origin and 150 meioses. DNA was extracted from blood samples using Chelex® resin, then purified using the Wizard® DNA Clean-Up System. Amplifications were performed using the PowerPlex® 16 System, the SGM Plus® kit and the GeneAmp® PCR System 9600. Amplified products were detected using an ABI PRISM® 377 DNA Sequencer. (3845)

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Clin. Can. Res. 9, 2981-2984. The RASSF1A tumor suppressor gene is commonly inactivated in adenocarcinoma of the uterine cervix 2003

Cohen, Y., Singer, G., Lavie, O., Dong, S.M., Beller, U., Sidransky, D.

Notes: The authors used real-time methylation-specific PCR to detect and quantitate the bisulfite-converted methylated version of the RASSF1A promoter region in uterine carcinoma samples. Bisulfite-treated DNA was purified using the Wizard® DNA Clean-Up System. (2710)

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Genetics 149, 1081-1088. Gene silencing by DNA methylation and dual inheritance in Chinese hamster ovary cells. 1998

Paulin, R. P., Ho, T., Balzer, H. J., Holliday, R.

Notes: Bisulphite-treated DNA was purified using the Wizard® DNA Clean-Up System prior to PCR. The amplified DNA was then cleaned using a Wizard® PCR Preps DNA Purification System prior to cloning and the cloned DNAs were sequenced using the fmol® DNA Cycle Sequencing System. (0001)

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Nucl. Acids Res. 22, 2990-2997. High sensitivity mapping of methylated cytosines. 1994

Clark, S. J. , Harrison, J. , Paul, C. L. , Frommer, M.

Notes: The Wizard® DNA Clean-Up System was used to desalt genomic DNA treated with sodium bisulfite to convert nonmethylated C to U. (1308)

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Nucl. Acids Res. 18, 1435-1439. Ligation-mediated PCR improves the sensitivity of methylation analysis by restriction enzymes and detection of specific DNA strand breaks. 1990

Steigerwald, S. D., Pfeifer, G. P., Riggs, A. D.

Notes: The Wizard® DNA Clean Up System was used to clean up DNA for methylation specific PCR. (0333)

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