Promega Corporation

Automated Genomic DNA Purification from Formalin-Fixed Paraffin-Embedded Tissue...

Automated Genomic DNA Purification from Formalin-Fixed Paraffin-Embedded Tissue Scientific Poster

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Abstract

Trista Schagat, Lynn Litterer, Melissa McCornack and Jeff Bacher
Promega Corporation, 2800 Woods Hollow Road, Madison, WI USA 53711-5399

DNA purification from formalin-fixed paraffin-embedded (FFPE) tissues can be time consuming and tedious. Here we demonstrate the utility of the Maxwell® 16 Instrument for automated purification of DNA from proteinase K-digested FFPE tissue samples. Using the prefilled cartridges of the Maxwell® 16 FFPE Tissue LEV DNA Purification Kit for Maxwell® 16, DNA can be extracted from up to 16 samples at a time, in approximately 30 minutes. Yields were comparable to a manual purification, and DNA was of suitable quality for multiplex real-time PCR and microsatellite instability analysis.

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  • Part# PS077
  • Printed in USA.

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