Steady-Glo® Luciferase Assay System Technical Manual

Literature # TM051

High-throughput quantitation of luciferase expression in mammalian cells is commonly performed by batch processing of 96- and 384-well plates. The Steady-Glo® Luciferase Assay System is designed for this purpose by providing long-lived luminescence when added to cultured cells. The homogeneous assay provides signal half-lives of over 5 hours in commonly used cell culture media without prior sample processing. Throughput rates of several thousand samples per hour may be achieved with high reproducibility under standard laboratory conditions.