MAO-Glo™ Assay Technical Bulletin

Literature # TB345

The MAO-Glo™ Assay provides a homogeneous luminescent method for measuring monoamine oxidase (MAO) activity from recombinant and native sources and measuring the effects of test compounds on MAO activities. The MAO-Glo™ Assay is performed by incubating the MAO enzyme with a luminogenic MAO substrate. The substrate of the MAO-Glo™ Assay is a derivative of beetle luciferin [(4S)-4,5-dihydro-2-(6-hydroxybenzothiazolyl)-4-thiazolecarboxylic acid]. MAO converts this luciferin derivative to methyl ester luciferin. After the MAO reaction is performed, the reconstituted Luciferin Detection Reagent is added to simultaneously stop the MAO reaction, convert the methyl ester derivative to luciferin and produce light. This addition initiates a stable glow-type luminescent signal with a half-life greater than 5 hours. The amount of light produced is directly proportional to MAO activity.