Automating nucleic acid purification from a variety of sample types is helpful for many high-throughput applications. Automated sample processing must be timely, precise and consistent, producing product that is free from cross-contamination, pure from other contaminants and of an expected yield for direct use in downstream applications. Here we present fast and easy-to-use nucleic acid isolation methods on the Eppendorf epMotion® 5075 VAC liquid handling workstation. The Wizard® SV 96 Genomic DNA Purification System and the SV 96 Total RNA Isolation System are used on the epMotion® 5075 VAC workstation to successfully isolate genomic DNA and total RNA from mouse tails and tissue culture cells with results comparable to manual and other automated isolation techniques.
Promega Notes 98, 12–15.
Sarah Shultz and Cristopher Cowan