Promega's pTargeT™ Vector has been constructed to improve the cloning and expression of DNA fragments amplified by PCR or modified by addition of deoxyadenosine tails. The presence of the alpha-peptide of beta-galactosidase in the pTargeT™ Vector allows detection of recombinants by blue/white screening, a feature not shared by other mammalian expression T-vectors. Comparison of the pTargeT™ Vector to another commercially available expression T-vector demonstrates enhanced mammalian expression of Renilla luciferase from the pTargeT™ Vector by as much as 15- to 18-fold.
Promega Notes 58, 2.
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