pTnT™ Vector

The pTnT™ Vector is designed for the convenient in vitro expression of cloned genes. Both SP6 and T7 polymerase promoters lie in tandem adjacent to the multiple cloning site. This permits gene expression from either an SP6- or T7-based coupled in vitro transcription/translation system. The presence of RNA phage promoters also allows for the highly efficient synthesis of RNA in vitro. The pTnT™ Vector also contains a 5´ β-globin leader sequence and synthetic poly(A)₃₀ tail, both of which have been shown to enhance expression of certain genes.

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The pTnT™ Vector is designed for the convenient in vitro expression of cloned genes. Both SP6 and T7 polymerase promoters lie in tandem adjacent to the multiple cloning site. This permits gene expression from either an SP6- or T7-based coupled in vitro transcription/translation system. The presence of RNA phage promoters also allows for the highly efficient synthesis of RNA in vitro. The pTnT™ Vector also contains a 5´ β-globin leader sequence and synthetic poly(A)₃₀ tail, both of which have been shown to enhance expression of certain genes.

Features - Benefits

• Flexible: The vector contains tandem SP6 and T7 phage promoters allowing use in the appropriate in vitro translation or transcription system.
• Convenient: Multiple cloning site provides a selection of restriction sites for cloning.

References

1. Falcone, D. and Andrews, D.W. (1991) Mol. Cell. Biol. 11, 2656–64.
2. Annweiler, A. et al. (1991) Nucl. Acids Res. 19, 3750.
3. Wakiyama, M. et al. (1997) Biochimie 79, 781–5.

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