Promega Corporation

Promoterless Firefly Luciferase Vectors

See All pGL4 Vectors
Promoterless firefly luciferase vectors are designed primarily to accept a putative promoter element for investigation of important regions controlling gene transcription. The promoterless vectors are available with three varieties of engineered firefly luciferase genes: luc2, luc2P or luc2CP. The luc2 gene is engineered to remove most cryptic transcription factor binding sites and improve mammalian expression through codon optimization. The luc2P and luc2CP and RapidResponse™ genes are luc2 genes appended with degradation sequences to influence the cellular half-life of the luc2 gene....

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pGL4.10[luc2] Vector

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pGL4.10[luc2] Vector

 Components

  • pGL4.10[luc2] Vector

    E665A1 x 20μg
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20μg
- E6651 CHF 612.00 Add to cart

pGL4.11[luc2P] Vector

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pGL4.11[luc2P] Vector

 Components

  • pGL4.11[luc2P] Vector

    E666A1 x 20μg
20μg
- E6661 CHF 612.00 Add to cart

pGL4.12[luc2CP] Vector

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pGL4.12[luc2CP] Vector

 Components

  • pGL4.12[luc2CP] Vector

    E667A1 x 20μg
20μg
- E6671 CHF 612.00 Add to cart

pGL4.14[luc2/Hygro] Vector

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pGL4.14[luc2/Hygro] Vector

 Components

  • pGL4.14[luc2/Hygro] Vector

    E669A1 x 20μg
20μg
- E6691 CHF 612.00 Add to cart

pGL4.15[luc2P/Hygro] Vector

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pGL4.15[luc2P/Hygro] Vector

 Components

  • pGL4.15[luc2P/Hygro] Vector

    E670A1 x 20μg
20μg
- E6701 CHF 612.00 Add to cart

pGL4.16[luc2CP/Hygro] Vector

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pGL4.16[luc2CP/Hygro] Vector

 Components

  • pGL4.16[luc2CP/Hygro] Vector

    E671A1 x 20μg
20μg
- E6711 CHF 612.00 Add to cart

pGL4.17[luc2/Neo] Vector

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pGL4.17[luc2/Neo] Vector

 Components

  • pGL4.17[luc2/Neo] Vector

    E672A1 x 20μg
20μg
- E6721 CHF 612.00 Add to cart

pGL4.18[luc2P/Neo] Vector

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pGL4.18[luc2P/Neo] Vector

 Components

  • pGL4.18[luc2P/Neo] Vector

    E673A1 x 20μg
20μg
- E6731 CHF 612.00 Add to cart

pGL4.19[luc2CP/Neo] Vector

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pGL4.19[luc2CP/Neo] Vector

 Components

  • pGL4.19[luc2CP/Neo] Vector

    E674A1 x 20μg
20μg
- E6741 CHF 612.00 Add to cart

pGL4.20[luc2/Puro] Vector

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pGL4.20[luc2/Puro] Vector

 Components

  • pGL4.20[luc2/Puro] Vector

    E675A1 x 20μg
20μg
- E6751 CHF 612.00 Add to cart

pGL4.21[luc2P/Puro] Vector

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pGL4.21[luc2P/Puro] Vector

 Components

  • pGL4.21[luc2P/Puro] Vector

    E676A1 x 20μg
20μg
- E6761 CHF 612.00 Add to cart

pGL4.22[luc2CP/Puro] Vector

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pGL4.22[luc2CP/Puro] Vector

 Components

  • pGL4.22[luc2CP/Puro] Vector

    E677A1 x 20μg
20μg
- E6771 CHF 612.00 Add to cart


You Also May Be Interested In

GenBank®/EMBL Accession Number

pGL4.10[luc2] Accession Number AY738222; pGL4.10[luc2] Vector sequence text file.
pGL4.11[luc2P] Accession Number AY738223; pGL4.11[luc2P] Vector sequence text file
pGL4.12[luc2CP] Accession Number AY738224; pGL4.12[luc2CP] Vector sequence text file.
pGL4.14[luc2/Hygro] Accession Number AY864928; pGL4.14[luc2/Hygro] Vector sequence text file.
pGL4.15[luc2P/Hygro] Accession Number AY864929; pGL4.15[luc2P/Hygro] Vector sequence text file
pGL4.16[luc2CP/Hygro] Accession Number AY864930; pGL4.16[luc2CP/Hygro] Vector sequence text file.
pGL4.17[luc2/Neo] Accession Number DQ188837; pGL4.17[luc2/Neo] Vector sequence text file
pGL4.18[luc2P/Neo] Accession Number DQ188838; pGL4.18[luc2P/Neo] Vector sequence text file.
pGL4.19[luc2CP/Neo] Accession Number DQ188839; pGL4.19[luc2CP/Neo] Vector sequence text file.
pGL4.20[luc2/Puro] Accession Number DQ188840; pGL4.20[luc2/Puro] Vector sequence text file.
pGL4.21[luc2P/Puro] Accession Number DQ188841; pGL4.21[luc2P/Puro] Vector sequence text file.
pGL4.22[luc2CP/Puro] Accession Number DQ188842; pGL4.22[luc2CP/Puro] Vector sequence text file.

Storage Conditions

Store at –20°C.

For product intended use please see Patents & Disclaimers tab.

pGL4.10[luc2] Vector.Figure 1. pGL4.10[luc2] Vector.
pGL4.11[luc2P] Vector.Figure 2. pGL4.11[luc2P] Vector.
pGL4.12[luc2CP] Vector. Figure 3. pGL4.12[luc2CP] Vector.
pGL4.14[luc2/Hygro] Vector.Figure 4. pGL4.14[luc2/Hygro] Vector.
pGL4.15[luc2P/Hygro] Vector.Figure 5. pGL4.15[luc2P/Hygro] Vector.
pGL4.16[luc2CP/Hygro] Vector.Figure 6. pGL4.16[luc2CP/Hygro] Vector.
pGL4.17[luc2/Neo] Vector.Figure 7. pGL4.17[luc2/Neo] Vector.
pGL4.18[luc2P/Neo] Vector.Figure 8. pGL4.18[luc2P/Neo] Vector.
pGL4.19[luc2CP/Neo] Vector.Figure 9. pGL4.19[luc2CP/Neo] Vector.
pGL4.20[luc2/Puro] Vector.Figure 10. pGL4.20[luc2/Puro] Vector.
pGL4.21[luc2P/Puro] Vector. Figure 11. pGL4.21[luc2P/Puro] Vector.
pGL4.22[luc2CP/Puro] Vector. Figure 12. pGL4.22[luc2CP/Puro] Vector.

Use Restrictions

E6651, E6661, E6671, E6691, E6701, E6711, E6721, E6731, E6741, E6751, E6761, E6771 For Research Use Only. Not for Use in Diagnostic Procedures.

Patents - Disclaimers

E6651, E6661, E6671, E6691, E6701, E6711, E6721, E6731, E6741, E6751, E6761, E6771 BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If the researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide the researcher with a full refund.
Researchers may use this product for research use only, no commercial use is allowed. "Commercial use" means any and all uses of this product and derivatives by a party for money or other consideration and may include but is not limited to use in: (1) product manufacture; and (2) to provide a service, information or data; and/or resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. Researchers shall have no right to modify or otherwise create variations of the nucleotide sequence of the luciferase gene except that researchers may: (1) create fused gene sequences provided that the coding sequence of the resulting luciferase gene has no more than four deoxynucleotides missing at the affected terminus compared to the intact luciferase gene sequence, and (2) insert and remove nucleic acid sequences in splicing research predicated on the inactivation or reconstitution of the luminescence of the encoded luciferase. No other use or transfer of this product or derivatives is authorized without the prior express written consent of Promega. In addition, researchers must either: (1) use luminescent assay reagents purchased from Promega for all determinations of luminescence activity of this product and its derivatives; or (2) contact Promega to obtain a license for use of the product and its derivatives. Researchers may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to the recipients and recipients agree to be bound by the terms of this label license. With respect to any uses outside this label license, including any diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA. This label license relates to Promega patents and/or patent applications on improvements to the luciferase gene.

E6651, E6661, E6671, E6691, E6701, E6711, E6721, E6731, E6741, E6751, E6761, E6771 U.S. Pat. No. 5,670,356.

E6651, E6661, E6671, E6691, E6701, E6711, E6721, E6731, E6741, E6751, E6761, E6771 Patent Pending.

E6651, E6661, E6671, E6691, E6701, E6711, E6721, E6731, E6741, E6751, E6761, E6771 U.S. Pat. No. 8,008,006 and European Pat. No. 1341808.

E6691, E6701, E6711, E6721, E6731, E6741, E6751, E6761, E6771 U.S. Pat. No. 7,728,118.

E6651, E6661, E6671, E6691, E6701, E6711, E6721, E6731, E6741, E6751, E6761, E6771 The method of recombinant expression of Coleoptera luciferase is covered by U.S. Pat. Nos. 5,583,024, 5,674,713 and 5,700,673. A license (from Promega for research reagent products and from The Regents of the University of California for all other fields) is needed for any commercial sale of nucleic acid contained within or derived from this product.

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