Steady-Glo® Luciferase Assay System

High-throughput quantitation of firefly (Photinus pyralis) luciferase expression in mammalian cells is commonly performed by batch processing of 96- and 384-well plates. Steady-Glo® Luciferase Assay System is designed for this purpose by providing long-lived luminescence when added to cultured cells. The homogeneous assay provides signal half-lives of over 5 hours in commonly used cell culture media without prior sample processing. Throughput rates of several thousand samples per hour may be achieved with high reproducibility under standard laboratory conditions.

Expand to Read More »

High-throughput quantitation of firefly (Photinus pyralis) luciferase expression in mammalian cells is commonly performed by batch processing of 96- and 384-well plates. Steady-Glo® Luciferase Assay System is designed for this purpose by providing long-lived luminescence when added to cultured cells. The homogeneous assay provides signal half-lives of over 5 hours in commonly used cell culture media without prior sample processing. Throughput rates of several thousand samples per hour may be achieved with high reproducibility under standard laboratory conditions.

Features - Benefits

• Greater Light Output: Greater assay sensitivity than other leading extended-lifetime firefly luciferase assay reagents.
• Improved Assay Precision and Reproducibility: Less sensitive to mixing conditions in multiwell plates. Particularly useful in 384-well plates.
• Convenience: Simply mix buffer with lyophilized substrate and add to cells in culture medium; no need to thaw or measure before use.
• No Sample Preprocessing: No need to remove culture medium or wash cells prior to adding assay reagent. Grow cells and assay them directly within the same multiwell plate.
• Easy to Use: Simply add reagent, which contains a cell lysis component, wait 5 minutes and measure luminescence.
• Robust: Compatible with many tissue culture media, including those containing up to 10% serum.
• Choose Your Configuration: Learn more about our custom options for this product at: www.promega.com/myway/

Applications

For more information, see the Protocols & Applications Guide.

Notes

Please contact Promega for information on bulk purchases.

« Collapse Content