Promega Corporation

ECL Western Blotting Substrate

The ECL Western Blotting Substrate is a highly sensitive non-radioactive, enhanced luminol-based chemiluminescent substrate for the detection of horseradish peroxidase (HRP) conjugates on immunoblots. The ECL Western Blotting Substrate detects and visualizes the presence of picogram (pg) amounts of antigen through the use of photographic or other suitable chemiluminescent imaging methods.

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ECL Western Blotting Substrate

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ECL Western Blotting Substrate

 Components

  • Peroxide Solution

    W100A1 x 125ml
  • Luminol Enhancer Solution

    W101A1 x 125ml
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  • Helix Refrigerator

This product is available through the Promega Helix onsite stocking program in a +4°C Helix Refrigerator. The program offers numerous convenient solutions to meet your lab's needs. The Helix Refrigerator is 5 cubic ft.


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250ml
- W1001 CHF 233.00 Add to cart

ECL Western Blotting Substrate

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ECL Western Blotting Substrate

 Components

  • Peroxide Solution

    W100B1 x 250ml
  • Luminol Enhancer Solution

    W101B1 x 250ml
500ml
- W1015 CHF 327.00 Add to cart


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Storage Conditions

Store at 2–8°C.

For product intended use please see Patents & Disclaimers tab.

Western blot (immunoblot) for β-actin in cytoplasmic lysate from HEK293T cells.Figure 1. Western blot (immunoblot) for β-actin in cytoplasmic lysate from HEK293T cells.

The blot was performed on a serial dilution of lysate; each lane contains an indicated amount of β-actin (ng) as quantitated via ELISA. Primary antibody (monoclonal) used at 1:5,000, Anti-Mouse IgG, HRP Conjugate secondary antibody (Cat.# W4021) used at 1:2,500; blot imaged with ECL Western Blotting Substrate (Cat.# W1015) and with 1-minute exposure.

Use Restrictions

W1001, W1015 For Research Use Only. Not for Use in Diagnostic Procedures.

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