Basics of PCR and Reverse–Transcription PCR Technologies (One day)

This presentation of polymerase chain reaction (PCR) theory and practice is ideal for nonscientists working in the field of Molecular Biology/Biotechnology or for scientists new to PCR who want to understand the fundamentals and gain laboratory experience.
Dates: February 7, April 2, June 11, October 3, 2013
Additional dates can be arranged by contacting PETAL with a custom training request.
Daily schedule:
9:30 to 17:30 (7 hr)
Fees:
400 € VAT excluded for researchers and technicians
300 € VAT excluded for students
Who should attend:
This class is designed for scientists, teachers, technicians, researchers and others who would like to learn more about setting up a PCR or RT-PCR experiment.
Maximum number of attendees: 10
A certificate of completion is given to participants.
Course Content
This course offers a one-day overview of PCR and RT-PCR technologies including a laboratory to optimize PCR reactions, analyze results and troubleshoot problems.
| Lectures |
Laboratory |
| Endpoint PCR concepts |
- PCR of DNA templates
- PCR purification
- Quantitation
|
| Reverse-transcription PCR |
RT-PCR of RNA templates to detect specific genes |
Specific course objectives may vary depending on individual and group interests.
Please contact us if you have questions.