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The Access RT-PCR System is designed for the reverse transcription (RT) and PCR amplification of a specific target RNA from either total RNA or mRNA. This one-tube, two-enzyme system provides sensitive, quick and reproducible analysis of even rare RNAs. The system uses AMV Reverse Transcriptase (AMV RT) from Avian Myeloblastosis Virus for first-strand DNA synthesis and thermostable Tfl DNA polymerase from Thermus flavus for second-strand cDNA synthesis and DNA amplification. The Access RT-PCR System includes an optimized single-buffer system that permits extremely sensitive detection of RNA transcripts, with...

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  • Prices valid for customers of Promega AG only
Product Size Conc. Catalog # *List Price Order QTY Add to Cart

Access RT-PCR Introductory System

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Access RT-PCR Introductory System

 Components

  • Upstream Control Primer

    A109B1 x 100μl
  • Downstream Control Primer

    A110B1 x 100μl
  • Positive Control RNA with Carrier

    A111A1 x 50μl
  • AMV Reverse Transcriptase

    A261B1 x 100u
  • AMV/Tfl 5X Reaction Buffer

    A808A1 x 1ml
  • dNTP Mix

    C114F1 x 20μl
  • MgSO4

    M197A1 x 1,250μl
  • Tfl DNA Polymerase

    M391A1 x 100u
  • Nuclease-Free Water

    P119E1 x 13ml
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  • This product can be added to a

  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


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20 reactions
- A1260 CHF 195.00 Add to cart

Access RT-PCR System

4.00
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Access RT-PCR System

 Components

  • Upstream Control Primer

    A109B1 x 100μl
  • Downstream Control Primer

    A110B1 x 100μl
  • Positive Control RNA with Carrier

    A111A1 x 50μl
  • AMV Reverse Transcriptase

    A261A1 x 500u
  • AMV/Tfl 5X Reaction Buffer

    A808A1 x 1ml
  • dNTP Mix

    C114A2 x 50μl
  • MgSO4

    M197A1 x 1,250μl
  • Tfl DNA Polymerase

    M391C1 x 500u
  • Nuclease-Free Water

    P119E1 x 13ml
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  • This product can be added to a

  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


Click here to learn more about the Helix Collection »


Already a Helix Customer?

Request to add this product to your Helix »


100 reactions
- A1250 CHF 756.00 Add to cart

Access RT-PCR System

4.00
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Access RT-PCR System

 Components

  • Upstream Control Primer

    A109B1 x 100μl
  • Downstream Control Primer

    A110B1 x 100μl
  • Positive Control RNA with Carrier

    A111A1 x 50μl
  • Access RT-PCR Core Reagents (100 reactions; A1250 minus Controls)

    A12814 x 1 each
  • AMV Reverse Transcriptase

    A261A1 x 500u
  • AMV/Tfl 5X Reaction Buffer

    A808A1 x 1ml
  • dNTP Mix

    C114A2 x 50μl
  • MgSO4

    M197A1 x 1,250μl
  • Tfl DNA Polymerase

    M391C1 x 500u
  • Nuclease-Free Water

    P119E1 x 13ml
500 reactions
- A1280 CHF 3 024.00 Add to cart


Available Separately

PRODUCT SIZE CONC CATALOG # *List Price ORDER QTY. Add to Cart

AMV Reverse Transcriptase

4.00
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AMV Reverse Transcriptase

 Components

  • AMV Reverse Transcriptase

    M510A1 x 300u
  • AMV RT 5X Buffer

    M515A1 x 500μl
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  • This product can be added to a

  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


Click here to learn more about the Helix Collection »


Already a Helix Customer?

Request to add this product to your Helix »


300u
10u/μl M5101 CHF 170.00 Add to cart

AMV Reverse Transcriptase

5.00
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AMV Reverse Transcriptase

 Components

  • AMV Reverse Transcriptase

    M510F1 x 1,000u
  • AMV RT 5X Buffer

    M515A3 x 500μl
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  • This product can be added to a

  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


Click here to learn more about the Helix Collection »


Already a Helix Customer?

Request to add this product to your Helix »


1,000u
10u/μl M5108 CHF 482.00 Add to cart

AMV Reverse Transcriptase (HC)

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AMV Reverse Transcriptase (HC)

 Components

  • AMV RT 5X Buffer

    M515A1 x 500μl
  • AMV Reverse Transcriptase (HC)

    M900A1 x 600u
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  • This product can be added to a

  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


Click here to learn more about the Helix Collection »


Already a Helix Customer?

Request to add this product to your Helix »


600u
20–25u/μl M9004 CHF 383.00 Add to cart

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Storage Conditions

Store all system components at –20°C. For long-term storage, the Positive Control RNA with Carrier must be stored at –70°C.

For product intended use please see Patents & Disclaimers tab.

Use Restrictions

A1260, A1250, A1280 For Research Use Only. Not for Use in Diagnostic Procedures.
M5101, M5108, M9004 For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.

Patents - Disclaimers

A1260, A1250, A1280 U.S. Pat. No. 6,242,235, Australian Pat. No. 761757, Canadian Pat. No. 2,335,153 and other patents and patents pending.

A1260, A1250, A1280 Use of this product for basic PCR is outside of any valid US or European patents assigned to Hoffman La-Roche or Applera. This product can be used for basic PCR in research, commercial or diagnostic applications without any license or royalty fees.

Access RT-PCR System

4.00

Tuesday, December 10, 2013 by Verónica Gonzalo Abril. Hospital Clínic Barcelona

Llevo mucho tiempo utilizando el producto y estoy contenta con los resultados. El único problema que he tenido, ha sido porque las enzimas venían con volumen insuficiente, pero los comerciales me arreglaron el problema de manera rápida y eficiente.

Access RT-PCR System

4.00

Tuesday, September 18, 2012 by Isabel García, Ciudad Real

Producto de una alta eficiencia, con un elevado precio pero en equilibrio con la calidad.

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