Promega Corporation

GloSensor™ cAMP Assay

The GloSensor™ cAMP Assay presents a novel approach to measuring cAMP levels in live cells. cAMP is a key second messenger involved in signal transduction of GPCRs acting through Gα-s and Gα-i proteins. The new assay is based on the GloSensor™ Technology, a genetically modified form of firefly luciferase into which a cAMP-binding protein moiety has been inserted. Upon binding of cAMP, conformational change is induced leading to increased light output. This live-cell assay excels at kinetic and modulation studies of signaling through cAMP.

Researchers can use the GloSensor™ cAMP Assay by transiently expressing...

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GloSensor™ cAMP HEK293 Cell Line

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GloSensor™ cAMP HEK293 Cell Line

 Components

  • GloSensor™ cAMP HEK293 Cell Line

    E126A2 x 1 vial
2 vials
- E1261 CHF 7 953.00 Add to cart

pGloSensor™-22F cAMP Plasmid

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pGloSensor™-22F cAMP Plasmid

 Components

  • pGloSensor™-22F cAMP Plasmid

    E230A1 x 20μg
20μg
- E2301 CHF 612.00 Add to cart

pGloSensor™-20F cAMP Plasmid

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pGloSensor™-20F cAMP Plasmid

 Components

  • pGloSensor™-20F cAMP Plasmid

    E117A1 x 20μg
20μg
- E1171 CHF 612.00 Add to cart

GloSensor™ cAMP Reagent

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GloSensor™ cAMP Reagent

 Components

  • GloSensor™ cAMP Reagent

    E129A1 x 25mg
25mg
- E1290 CHF 500.00 Add to cart

GloSensor™ cAMP Reagent

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GloSensor™ cAMP Reagent

 Components

  • GloSensor™ cAMP Reagent

    E129B1 x 250mg
250mg
- E1291 CHF 2 000.00 Add to cart


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GenBank®/EMBL Accession Number

pGloSensor™-20F cAMP, EU770615; pGloSensor™-20F cAMP Vector Sequence (.txt file)
pGloSensor™-22F cAMP, GU174434; pGloSensor™-22F cAMP Vector Sequence (.txt file)

Storage Conditions

Store the pGloSensor™ cAMP Plasmid at –20°C and the GloSensor™ cAMP Reagent at –70°C. Store the resuspended GloSensor™ cAMP Reagent at –70°C in single-use aliquots.

For product intended use please see Patents & Disclaimers tab.

Overview of the GloSensor cAMP Assay protocol.Figure 1. Overview of the GloSensor™ cAMP Assay protocol.

Cells are pre-equilibrated with GloSensor™ Reagent for approximately two hours and then treated with specific agonists/antagonists or library of compounds, and luminescence is measured after 15–30 minutes.

Cells are pre-equilibrated with GloSensor™ Reagent for approximately two hours and then treated with specific agonists/antagonists or library of compounds, and luminescence is measured after 15–30 minutes.

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Interrogation of G-alpha-s-coupled receptors using the GloSensor cAMP Assay.Figure 2. Interrogation of G-alpha-s-coupled receptors using the GloSensor™ cAMP Assay.

The GloSensor™ cAMP HEK293 Cell Line was treated with varying concentrations of agonists or antagonists of the endogenous beta2-adrenergic receptor. Panel A. Cells treated with agonists. Panel B. Cells treated with antagonists in the presence of 1µM metaproterenol agonist. Experiments were performed as described in Section 5 of Technical Manual #TM076.

The GloSensor™ cAMP HEK293 Cell Line was treated with varying concentrations of agonists or antagonists of the endogenous beta2-adrenergic receptor. Panel A. Cells treated with agonists. Panel B. Cells treated with antagonists in the presence of 1µM metaproterenol agonist. Experiments were performed as described in Section 5 of Technical Manual #TM076.

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pGloSensor-22F cAMP Plasmid.Figure 3. pGloSensor™-22F cAMP Plasmid.

Use Restrictions

E1261, E2301, E1171, E1290, E1291 For Research Use Only. Not for Use in Diagnostic Procedures.

Patents - Disclaimers

E1261, E2301, E1171 BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
Researchers may use this product in their own research solely for the luminescent determination of molecular binding, enzymatic activity or other functional activity. Researchers shall have no right to modify or otherwise create variations of the nucleotide sequence encoding the GloSensor™ technology of this product (i.e., the bioluminescent chimeric protein comprising luciferase and other functional domains). Researchers may transfer derivatives to others provided that recipients agree to be bound by the terms of this label license prior to such transfer. No other use or transfer of this product is authorized without the prior express written consent of Promega. In addition, researchers must either: (1) use GloSensor™ cAMP Reagent for in vitro and in cell applications, and VivoGlo™ Luciferin, In Vivo Grade, for animal applications for all determinations of luminescence activity of this product and derivatives; or (2) contact Promega to obtain a license for use of the product and derivatives in conjunction with luminescent assay reagents not purchased from Promega. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.

E2301, E1171 U.S. Pat. No. 5,670,356.

E2301, E1171 Patent Pending.

E2301, E1171 U.S. Pat. No. 8,008,006 and European Pat. No. 1341808.

E2301, E1171 U.S. Pat. No. 7,728,118 and other patents pending.

E1261 Commercial use of this cell line requires a license from AdVec Inc.

E1261 Use of Genetically Modified Microorganisms (GMM)
Information for European Customers: These products are genetically modified as described in Promega technical literature. As a condition of sale, use of this product must be in accordance with all applicable local guidelines on the contained use of genetically modified microorganisms, including the Directive 2009/41/EC of the European Parliament and of the Council.

E1261 HEK293 cells were obtained under license from AdVec Inc.

E1261, E2301, E1171 The method of recombinant expression of Coleoptera luciferase is covered by U.S. Pat. Nos. 5,583,024, 5,674,713 and 5,700,673. A license (from Promega for research reagent products and from The Regents of the University of California for all other fields) is needed for any commercial sale of nucleic acid contained within or derived from this product.

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