I-PpoI (Intron-Encoded Endonuclease)

Product	Size	Conc.	Catalog #	*List Price	Order QTY	Add to Cart
I-PpoI Protocol MSDS Components Add to Helix Close Window I-PpoI  Components Bovine Serum Albumin, Acetylated R396A1 x 1,000μl I-PpoI R703A1 x 10,000u I-PpoI Positive Control Plasmid R707A1 x 10μg I-PpoI 10X Buffer R994A1 x 1ml Close Window This product can be added to a Helix Freezer This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft. Click here to learn more about the Helix Collection » Already a Helix Customer? Login to request to add this product to your Helix »	10,000u	100–200u/μl	R7031	Fr. 179.00		Add to cart

You Also May Be Interested In

• LigaFast™ Rapid DNA Ligation System

• Blue/Orange Loading Dye, 6X

• Agarose, LE, Analytical Grade

• Ethidium Bromide Solution, Molecular Grade

Frequency of Cutting

No sites are present in any of the commonly used plasmids or vectors. One site is present in chromosome 12 of S. cerevisiae DNA.

Incubation Conditions

I-PpoI Buffer. 37°C.

Percent Activity in 4-CORE^® Buffer

A	B	C	D	I-PpoI
10–25%	25–50%	25–50%	25–50%	100%

Note: A precipitate may be observed after thawing. If detected, heat to 37°C for 3–5 minutes and mix to dissolve. The buffer may appear cloudy after heating; this will not affect the performance of the product.

Source

I-PpoI is an intron-encoded endonuclease from Physarum polycephalum. The enzyme is produced from a recombinant E. coli strain.

Storage Buffer

50mM Tris-HCl (pH 7.5), 0.01% NP-40, 50mM NaCl, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.

Storage Conditions

Store at –20°C.

For product intended use please see Patents & Disclaimers tab.

Use Restrictions

R7031 For Research Use Only. Not for Use in Diagnostic Procedures.