Prime-a-Gene® Labeling System

The Prime-a-Gene® Labeling System provides a complete set of complementary reagents, including Labeling 5X Buffer that contains random synthetic hexadeoxynucleotide primers for random-primed labeling of linear template DNA with radionucleotides. As little as 25ng of input DNA can be used to generate probes with specific activities >1 × 10⁹cpm/μg.

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The Prime-a-Gene® Labeling System provides a complete set of complementary reagents, including Labeling 5X Buffer that contains random synthetic hexadeoxynucleotide primers for random-primed labeling of linear template DNA with radionucleotides. As little as 25ng of input DNA can be used to generate probes with specific activities >1 × 10⁹cpm/μg.

Features - Benefits

• Ready to Use: Includes reagents needed for random-primed labeling of linear DNA, including random synthetic hexadeoxynucleotide primers (excluding radionucleotides).
• High Specific Activity: Probes with specific activities >1 × 10⁹cpm/μg can be generated.

Applications

• Detection of single-copy genes and rare sequences.
• Southern, Northern and dot blotting.
• Plaque and colony lift hybridizations.

For more information, see the Protocols & Applications Guide.

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