Protein Phosphatase Assays

ProFluor™ Phosphatase Assays

The ProFluor^® Phosphatase Assays overcome safety issues associated with radioactive assays while maintaining sensitivity, specificity and simplicity required for screening chemical libraries for novel inhibitors of protein phosphatases. These assays are robust with Z´ factor values routinely greater than 0.8.

• Ser/Thr PPase Assay Details + Ordering
• Tyr PPase Assay Details + Ordering

Colorimetric Phosphatase Assays

Both the Tyrosine Phosphatase and the Serine/Threonine Phosphatase Assay Systems detect the release of phosphate from specific peptide substrates by measuring the appearance of a phosphate complex of molybdate:malachite green. Each system includes ready-to-use, specific substrates.

• Product Details + Ordering

Protein Phosphatases

PPase-2A is a serine/threonine phosphatase isolated from human red blood cells. It is isolated as the heterodimer of 60kDa (A) and 36kDa (C) subunits. It has the ability to dephosphorylate the α-subunit of phosphorylase kinase. PPase-2B is a heterodimeric enzyme composed of a 19kDa calcium-binding subunit and a catalytic subunit (61kDa) that binds calmodulin.

• PPase-2A Details + Ordering
• PPase-2B Details + Ordering

This chapter of the Protocols and Applications Guide describes the molecular basis of cell signaling and the various assays used to study cell signaling.

This video covers a procedure for culturing and plating cells for use in a cell-based assay such as CellTiter-Glo^® Luminescent Cell Viability Assay.