Anti-ACTIVE® JNK pAb, Rabbit, (pTPpY)

Anti-ACTIVE® JNK pAb is a polyclonal antibody from rabbit serum. The antibody is affinity purified using a dually phosphorylated peptide that corresponds to the active form of the JNK enzymes.

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Anti-ACTIVE® JNK pAb is a polyclonal antibody from rabbit serum. The antibody is affinity purified using a dually phosphorylated peptide that corresponds to the active form of the JNK enzymes.

Features - Benefits

• Specificity: Preferentially detects the dually phosphorylated, active form of the stress-activated protein kinase (SAPK), also known as c-Jun N-terminal kinase, JNK.
• Immunogen: Dually phosphorylated Thr/Pro/Tyr region (pTPpY) derived from the catalytic core of the active form of JNK kinase, which corresponds to Thr¹⁸³ and Tyr¹⁸⁵ of the mammalian JNK2 enzyme.
• Antibody Form: Affinity-purified rabbit IgG; supplied in 10mM sodium phosphate (pH 7.4), 20mM NaCl.
• Value: Anti-ACTIVE® JNK pAb is available in two convenient sizes. Cat.# V7931 will generate up to 200ml of blotting solution, sufficient for 20 Western blots of 10ml each. The larger size, Cat.# V7932, will generate up to 600ml of blotting solution, sufficient for 60 Western blots of 10ml each.

Applications

(Suggested working dilution)

• Western blotting (1:5,000).
• Immunocytochemistry (1:1,000).
• Immunohistochemistry (1:500).

For more information, see the Protocols & Applications Guide.

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